Coding

Part:BBa_K3044024:Design

Designed by: Catharina Bang Jensen   Group: iGEM19_SDU-Denmark   (2019-10-10)


Codon optimized Cas9 for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutations to activate the catalytically domains RuvC and HNH were made by A10D and A840H mutations, respectively. The point mutations made are c.29C>A, c.30G>T for the RuvC domain and c.2518C>G, c.2519C>A, c.2520G>T for the HNH domain.

These mutations are codon optimized according to a codon frequency table [1]


Source

The mutations made to activate the catalytic domains are A10D and A840H in part BBa_K3044008.

References